A new member of the VG series, VG-25 4D, is packed with 2.5 μm packing material, smaller than the packing material used in the existing product, VG-50 4D. VG-25 4D showed significantly higher theoretical plate number on all four sugars analyzed over VG-50 4D.
VG-25 4D also exhibited higher separation ability over other manufacturer’s amino column packed with 3 μm particles.
Sample: 5 μL
5 mg/L each
- 1.Fructose
- 2.Mannose
- 3.Glucose
- 4.Sucrose
理論段数比較
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| VG-25 4D | VG-50 4D | Silica-based amino column | |
|---|---|---|---|
| Fructose | 16,900 | 6,700 | 13,800 |
| Mannose | 18,500 | 7,900 | 13,900 |
| Glucose | 11,600 | 3,700 | 5,200 |
| Sucrose | 19,500 | 7,800 | 16,600 |
- Column
- ：Shodex HILICpak VG-25 4D, VG-50 4D, Silica-based amino column from other manufacturer (4.6 mm I.D. x 150 mm each)
- Eluent
- ：CH3CN/H2O=80/20
- Flow rate
- ：0.6 mL/min
- Detector
- ：RI
- Column temp.
- ：40 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Related Products
- Polymer-based Columns for Reversed Phase Chromatography (ODP2 HP)
- Polymer-based Columns for Reversed Phase Chromatography (Asahipak)
- Polymer-based Columns for Reversed Phase Chromatography (RSpak)
- Polymer-based Columns for Hydrophilic Interaction Chromatography (HILIC) (HILICpak)
- Polymer-based Columns for Hydrophilic Interaction Chromatography (HILIC) (Asahipak)
- Silica-based Reversed Phase Chromatography Columns (ODS Columns)
- Silica-based Reversed Phase Chromatography Columns (ODS Columns for UHPLC)
Product Information (Related Information)
- Eluent Conditions for RSpak Series
- Eluent Conditions for Asahipak (ODP, C4P, NH2P)
- Repeatability for ODP-50 4E, A Polymer-based Column for Reversed Phase Chromatography, under Alkaline Conditions
- Features of RSpak DE Series
- Features of HILICpak VG-50 Series
- Advantage of ODP2 HP
- Performance of ODP2 HP