Features of HILICpak VG-50 Series (1) (Recovery of Reducing Sugar)

HILICpak VG-50 shows high recovery ratio of reducing sugars, such as mannose. Detection of reducing sugars in low concentration is possible with VG-50.  The silica-based amino column shows low recovery ratio of reducing sugars due to the interaction of the primary amino group on packing material and the carbonyl group of reducing sugar.  The formation of a Schiff base causes reducing sugars to be adsorbed to the packing material.

Sample : 5 mg/mL each, 5 μL
1. Fructose
2. Mannose
3. Glucose
4. Sucrose

Columns      : Shodex HILICpak VG-50 4D (4.6 mm I.D. x 150 mm)
               Silica based amino columns from other manufacturers
               (4.6 mm I.D. x 150 mm each)
Eluent       : H2O/CH3CN=20/80
Flow rate    : 0.6 mL/min (VG-50 4D)
               1.0 mL/min (Silica-based amino column)
Detector     : RI
Column temp. : 40 °C

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index

Applications (Related Information)