ODP2 HP does not adsorb protein, because the surface of the packing material is high polar and the pore size is small. ODS columns showed pressure rise as increasing injection times, because BSA is adsorbed packing material. However ODP2 HP showed stable pressure during repeat injections of BSA sample.
Sample: 7.0 mg/mL BSA, 5 μL
*Considering original pressure as 100 %
- Columns
- ：Shodex ODP2 HP-2B, ODS from other manufacturer (2.0 mm I.D. x 50 mm each)
- Eluent
- ：1 mM Ammonium acetate aq./CH3CN=90/10
- Flow rate
- ：1.0 mL/min
- Detector
- ：UV (220 nm)
- Column temp.
- ：43 ℃
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.