lHPLCSystemTroube ShootingUnstable baselineNoiseYESYESYESYESYESYESYESYESYESYESNONOYESYESSTARTSTARTNONOYESYESNONONONONONONONONONOYESYESNONONONOColumn PressurenormalBaseline stable when pump stoppedLeakage observedFlow rate ■uctuationTighten connectionsBaseline stable when pump stoppedOn-line degasser usedSpike noise found from beginningNoise shape leadingBubbles in ■ow cell······Main causeMonotonic drift······Main causeIrregular noiseSpike noiseAir in columnColumn temperature exceeding eluent boiling point(with different solvent, salt or others)YESYES*: Do not apply backpressure to detector······Possible causeMalfunction of check valveTemperature ■uctuationDeterioration of detector lampInsuf■cient groundingMalfunction of recorderBubbles in ■ow cellContamination of ■ow cellInsuf■cient eluent replacementContamination of columnPulsation of pumpDeterioration of eluent······Possible causeInsuf■cient groundingDeterioration of detector lampMalfunction of integratorInsuf■cient degassing eluentProblem solved by applying backpressure to column*Contamination of ■ow cell······Solution· Replace check valve· Clean check valve · Warm up detector suf■ciently· Shield detector from wind· Check column oven· Check detector temperature controlReplace lampEstablish suf■cient groundingRepair recorderWash inside of detectorWash inside of detectorWash ■ow line of HPLC· Wash column· Check column performance· Replace columnUse damper· Use eluent containing stabilizer (for THF, use THF containing BHT)······SolutionEstablish suf■cient groundingReplace detector lampRepair integratorUse on-line degasser· Check column performance· Replace columnReduce temperature of column oven and/or post-column reactioncoilWash ■ow cellRepair detectorOKCommon causes for abnormal chromatograms71HPLC System Trouble ShootingHPLC System Trouble Shooting
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