ll ABCDetectorCoumnTroube ShootingAbnormal peak shapesPressure elevationNONOYESYESYESYESYESYESYESYESSTARTPeak tailingPeak split,shouldersBroad peakGhost peakNo peakPeakleadingYESYESNONONONONONONONONONOYESYESNONOYESYESNONONONOYESYESYESYESNONOSalt precipitated in the eluentPressure still high when C is disconnectedPressure still high when B is disconnectedPressure still high when A is disconnectedUsed line ■lterLine ■lter blocked······Main causeSelection of column and/or eluent appropriateProblem solved by reducing injection volumePumpPressure still high after washingYESYESColumnblockedScreen eluent and sample through 0.45 µm ■lterInjector blockedPump piping blocked······Possible causeStrong sample retentionVoid existing at top of columnAdsorption of impurities in columnChanneling in columnLeading caused by sample properties (ionic and micellar properties)· Adsorption of impurities in column· Deterioration of columnSample overloadContamination Impurities existing in eluentSystem trouble· Eluent not ■owing· Injector at wrong position (load/inject)· Detector deactivatedSample adsorbed in column· Insuf■cient column equilibrationColumnInjectorSalting outDetector piping blockedPressure under maximum column pressureColumn deteriorated······SolutionChange column and/or eluentReplace column· Wash column· Check column performance· Replace columnReplace columnChange column and/or eluent· Wash column· Check column performance· Replace columnReduce injection volumePrepare fresh sampleWash system ■ow lineReplace eluentCheck systemRepeat sample injection······Main cause······Possible cause······Solution· Change eluent composition· Wash system before analysis· Clean detector· Replace piping· Check column performance· Replace columnColumn end-■ttingblocked· Clean injector· Replace injector· Replace ■lter in pump· Clean pump· Replace pump piping· Wash line ■lter· Replace line ■lterReplace column70Column Trouble ShootingColumn Trouble ShootingCommon causes for abnormal chromatograms
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