Shodex_HPLC_Columns_2023-2024

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4321134232410505342123413400505052152413600+N81234567005i l l214350Column Eluent Flow rate Detector Column temp. : 50 ˚C910+N+NN+H3C Flow rate AnonExchange Chromatography Coumns / CationExchangeChromatography CoumnsSample : Human serum 0.5 %, 200 µL1. IgG2. Transferrin3. (Unknown)4. Serum albumin15Sample : Gibberellin1. endo GA14CH3HOHOOCCOOH10Data provided by Prof. Yamaguchi,Faculty of Agliculture, University of Tokyo.ParaquatCH32Cl-10: Shodex IEC QA-825Column : (A); 20 mM Tris-HCl buffer (pH8.6) Eluent (B); (A) + 0.5 M NaCl Linear gradient; 100 % (A) to 50 % (B), 60 min : 1.0 mL/min Flow rate Detector : UV (280 nm) Column temp. : Room temp. : Shodex Asahipak ES-502N 7C : CH3COOH/H2O/CH3OH = 0.1/0.4/99.5: 1.5 mL/min: UV (210 nm)Column Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex Asahipak ES-502C 7C: 50 mM Sodium phosphate buffer (pH7.0) + 150 mM NaCl: 1.0 mL/min: UV (288 nm)3045 min2. GA14CH3HOHOOCCOOH20 minSample : 20 µLDiquat2Br-20 minColumn Eluent 101520min101520minSample : 90 µL1. Myoglobin2. Trypsinogen 3. Ribonuclease A4. α-Chymotrypsinogen A 5. Cytochrome c 6. Lysozyme153045 min10101520min101520minSample : 5 µL0.16 %1. Trypsinogen 2. α-Chymotrypsinogen A 0.08 % 3. Ribonuclease A 0.12 % 4. Cytochrome c 0.12 %5. Lysozyme 0.06 %0.51315171412111615202530354045Sample : 100 µL1. Conalbumin 0.1 % 2. Transferrin 0.2 % 3. Ovalbumin 0.2 % 4. Trypsin inhibitor 0.2 % 1,2101520min1.01.5 min1. Asp2. Thr3. Ser4. Glu5. Pro6. Gly7. Ala8. Cystine9. Val10. Met11. Ile12. Leu13. Tyr14. Phe15. Lys16. NH317. His18. Arg1850minProteins in human serumGibberellin isomersParaquat and diquatProtein separation using cation exchange columnsEffects of eluent pH on DEAE-825 analysisUltra-rapid analysis of hemoglobinsStandard amino acidspH6.0pH8.0Column Eluent Flow rate Detector Column temp. : 25 ˚C: Shodex IEC DEAE-825 : (A); 20 mM Piperazine-HCl buffer (pH6.0), 20 mM Bis-Tris-HCl buffer (pH7.0) 20 mM Tris-HCl buffer (pH8.0), 20 mM Ethanolamine-HCl buffer (pH9.0) 20 mM 1,3-Diaminopropane-HCl buffer (pH10.0) (B); (A) + 0.5 M NaCl Linear gradient; (A) to (B), 20 min: 1.0 mL/min: UV (280 nm): Shodex IEC CM-825: (A); 20 mM Sodium phosphate 　 buffer (pH7.0) (B); (A) + 0.5 M NaCl Linear gradient; (A) to (B), 60 min: 1.0 mL/minFlow rate Detector : UV (280 nm)Column temp. : Room temp.: Shodex CXpak P-421S: MCI BUFFERTM PH Kit (Mitsubishi Chemical Corporation) Low pressure step gradient; Column Eluent PH-1 (0 min), PH-2 (0.2 min), PH-3 (13.5 min), PH-4 (23.2 min), PH-RG (47.0 min): Ninhydrin Coloring Solution Kit for HITACHI (FUJIFILM Wako Pure Chemical Corporation)R1:R2 = 50:50 Reagent : (Eluent) 0.5 mL/min (Reagent) 0.35 mL/min: VIS (570 nm)Detector Column Temp. : 63 ˚CReaction Temp. : 120 ˚CpH7.0pH9.0Column Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex IEC SP-FT 4A: (A); 20 mM MES buffer (pH5.6) (B); (A) + 0.5 M Na2SO4 Linear gradient; (A) to (B), 2 min: 1.7 mL/min: UV (280 nm) pH10.063