)( thgew )ll( thgew0 0 F-2OEP/GEP0 F-50 nauuP illillGHQGF-31HQGF-21HQGF-7M HQillGF-51HQGF-7M HQGF-3G1HQHQ3456789345678923105053421564←56123465124356123400010 rauceoM10 rauceoM56 Aqueous-Organc SECCoumnsColumn Eluent Flow rate Detector Column temp. : 30 ˚CPolymers having both hydrophobic and hydrophilic functional groups may exhibit hydrophobic interactions with packing materials.When analyzing such polymers, addition of organic solvents to the eluent can suppress the hydrophobic interaction.Column Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex Asahipak GF-510 HQ : 50 mM LiCl aq./CH3CN = 60/40: 0.6 mL/min: UV (254 nm)GF-210 HQ demonstrates an improved separation of low molecular substances. The chromatograms below show that the peaks obtained by GF-210 HQ are separated with deeper notches compared to peaks obtained by GF-310 HQ. GF-210 HQ is capable of separating oligosaccharides (trisaccharides to hexasaccharides) while those oligosaccharides were eluted all together when analyzed by GF-310 HQ.Column Eluent Flow rate Detector Column temp. : 50 ˚C: Shodex Asahipak GF-210 HQ x 2 Shodex Asahipak GF-310 HQ x 2: H2O: 0.6 mL/min: RI104107106105103102Mw : 8,600Mw : 48,600Mw : 149,000101520: Shodex Asahipak GF-HQ series: H2O: 0.6 mL/min: RI1011Elution volume (mL)Sample : 0.2 % each, 50 µLSodium poly(styrene sulfonate)CH2 CH SO3Na1520Sample : 0.1 % each, 20 µL1. Maltohexaose2. Maltopentaose3. Maltotetraose4. Maltotriose5. Maltose6. Glucose1~32530Sample : Poly(lactic-co-glycolic acid) 0.02 %, 200 µLn 25 min20 min35 minColumn Eluent Flow rate Detector Column temp. : 40 ˚C(A) THFColumn Eluent Flow rate Detector Column temp. : 30 ˚CColumn Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex Asahipak GF-HQ series: DMF: 0.6 mL/min: RI: Shodex Asahipak GF-7M HQ : CH3CN: 0.6 mL/min: RI(B) Chloroform: Shodex Asahipak GF-510 HQ + GF-310 HQ: (A); THF, (B); Chloroform, (C); DMF: 0.5 mL/min: (A),(B) UV (254 nm), (C) UV (270 nm)104107106105103102Elution volume (mL)1015Sample : Polystyrene 1 mg/mL each, 50 µL1. Mw : 1,090,0002. Mw : 190,00037,9003. Mw : 9,1004. Mw : 2,9805. Mw : 6. Mw : 50020 min01112025min20 minCalibration curves for GF-HQ series using pullulan (eluent: H2O)Sodium polystyrene sulfonatesComparison of two GF column performances for the separation of maltooligosaccharidesGF-210 HQ×2GF-310 HQ×2Calibration curves for GF-HQ series using PEG/PEO (eluent: DMF)Comparison of polystyrene separation under three different solvent conditionsBiodegradable Polymer(C) DMF47
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