)lillGSQGSQ456943210500032514786905lll( thgew rauceoM-32-22nauuP0 H0 H Multimode CoumnsΣfColumn Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex Asahipak GS-HQ series: H2O: 0.6 mL/min: RIPurine in food is analyzed as purine base after steps of sample preparation; homogenization, freeze drying, hydolyzation with 70 % perchloric acid, and neutralization. Example below shows the analysis of purine in regular beer and beer treated with guanase (an enzyme that degrades guanine to xanthine). The following data indicate that guanine was decreased and xanthine was increased by guanase.Column Eluent Flow rate Detector Column temp. : 35 ˚C: Shodex Asahipak GS-320 HQ: 150 mM Sodium phosphate buffer (pH2.5): 0.6 mL/min: UV (260 nm)GS-220 HQ allows to elute monosaccharides, disaccharides, and sugar alcohols after the indigestible component fraction (indicated by an arrow on the chromatogram).This separation makes the method preferable for the quanti■cation of low molecular weight water-soluble dietary ■ber.Column Eluent Flow rate Detector Column temp. : 60 ˚C: Shodex Asahipak GS-220 HQ x 2: H2O: 0.5 mL/min: RI10710610510410310278Elution volume (mL)adenineguaninehypoxanthine1020Sample : 20 µLLow molecular weight water soluble dietary ■ber101520101112xanthineuric acid304050 minI.S.(Glycerol)253035404550minGS-HQ columns work not only under SEC (GFC) mode, but also under multimode, adding hydrophobic and ionic interactions. By carefully selecting the eluent, they provide separation mode that was not available with other types of columns. GS-320 HQ shows excellent performance separating hydrophilic peptides, particularly for acidic and basic peptides. Column Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex Asahipak GS-320 HQ: 30 mM Ammonium acetate buffer (pH6.7): 0.5 mL/min: UV (220 nm)Column Eluent Flow rate Detector Column temp. : 40 ˚CMWplGlu-Ala-GluArg-AspGly-His-LysArg-Pro-Lys-ProΣf : Hydrophobic parameterpl : Isoelectric point3472893404973.126.759.9511.44guaninexanthine102030400.390.680.293.2410152050 min1020Data provided by Kiyoko Kaneko Ph.D.,Faculty of Pharmaceutical Sciences, Teikyo University10152025: Shodex Asahipak GS-320 HQ: 10 mM NaH2PO4 aq./10 mM Na2HPO4 aq. = 1000/31: 1.0 mL/min: UV (260 nm)Sample : 20 µL0.025 %1. Glu-Ala-Glu 0.05 %2. Arg-Asp 3. Gly-His-Lys 0.025 %4. Arg-Pro-Lys-Pro 0.025 %2530 minxanthine304050 minSample : 50 µg/mL each, 20 µL1. IMP2. GMP3. AMP4. Inosine5. Hypoxanthine6. Guanosine7. Guanine8. Adenosine9. Adenine303540minLow molecular weight water-soluble dietary fiberCalibration curves for GS-HQ series using pullulanPurine base standards Purine bases in beerPeptides“Umami”Normal beerGuanase treated beer45
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