)( thgew )lillL05LB-806L04LB-806ML03LB-80illL05LB-806LB-806ML04LB3LB-805456789456789i i005llllll( thgew2.5 rauceoM rauceoM )( oitar esonot langSN/S)l( ssaM)l( ssaMom/gom/g raoM raoM () B-8B-8-80OEP/GEPB-8B-82.5B-8nauuP Aqueous SECGFCCoumns: Poymer-based5,3001,420Copolymer 7:3Mn : 2,000Mw : 14,400Mw/Mn : 7.40Copolymer 3:7Mn : 6,400Mw : 28,900Mw/Mn : 4.53Mn : 58,790Mw : 166,200Mw/Mn : 2.83Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex OHpak SB-806M HQ x 2: 20 mM LiBr in DMF: 1.0 mL/min: RIThe OHpak LB-800 series is able to detect low molecular weight substances owing to its improved low baseline noise level while using it with a multiangle light scattering detector. This cannot be achieved with other manufacturer's SEC column. LB-806M (8.0 mm I.D. x 300 mm)SB-806M HQ (8.0 mm I.D. x 300 mm)SEC column from other manufacturer (7.8 mm I.D. x 300 mm)Column Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex OHpak LB-806M x 2: 0.1 M NaNO3 aq.: 1.0 mL/min: RI MALS (Multi angle light scattering)Sample : 100 µLPoly(1-vinylpyrrolidone-co-vinyl acetate) 0.1 % each1015Molecular weight was determined from the calibration curve of PEG/PEO.1.0x1081.0x1071.0x1061.0x1051.0x1041000.0100.010.01.010.01071061051041031022025minSample : Pullulan (MW : 12,000) 0.5 %, 10 µL10Sample : Sodium alginate 0.1 %, 100 µL15.0Time (min)Column Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex OHpak LB-800 series: H2O : 1.0 mL/min: RIColumn Eluent Flow rate Detector Column temp. : 30 ˚C0111Elution volume (mL)15 min1.0x1081.0x1071.0x1061.0x1051.0x1041000.0100.010.020.01.0Mn : 12,070Mw : 15,220Mw/Mn : 1.26Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex OHpak LB-800 series: DMF: 1.0 mL/min: RILB-806M (8.0 mm I.D. x 300 mm)SB-806M HQ (8.0 mm I.D. x 300 mm)SEC column from other manufacturer (7.8 mm I.D. x 300 mm)Column Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex OHpak LB-806M Shodex OHpak SB-806M HQ SEC column from other manufacturer: 0.1 M NaNO3 aq.: 1.0 mL/min: MALS (Multi angle light scattering) (90 °): Shodex OHpak LB-806M x 2: 0.1 M NaNO3 aq. : 1.0 mL/min: RI MALS (Multi angle light scattering)1071061051041031022125020015010050Molecular weight (Pullulan) Sample : Sodium heparin 0.1 %, 100 µL15.010.0Time (min)101112Elution volume (mL)20.0CopovidonesSodium alginateComparison of pullulan detection using multi angle light scattering detectorCalibration curves for LB-800 seriesusing pullulan (eluent: H2O)Sodium heparinCalibration curves for LB-800 series using PEG/PEO (eluent: DMF)12,00020,80043
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