5 4 3 -80OEP/GEPB-84 3 2.5 B-8nauuP () ( thgewll)lillSB-8S06 HQHSB-8SQSB-80HQ0HQS0HQQSB-806M HQ)illSB-80HQSB-80HQSBQSB-8SB-80H06 HQQSB-806M HQ456945678955l5ll( thgew rauceoM05 rauceoM2.5 HB-802 H)l( ssaMom/g raoM Aqueous SECGFCCoumns: Poymer-basedColumn Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex OHpak SB-800 HQ series: H2O: 1.0 mL/min: RIFor the analysis of cationic polymers, such as polyallylamine, the polymer is observed to adsorb on the column or delayed in elution when low sodium nitrate eluent was used. These phenomena can be suppressed by increasing the concentration of sodium nitrate in the eluent. In the case of polyallylamine, a good shape chromatogram is obtained when sodium nitrate concentration is 0.1 M or higher.Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex OHpak SB-806M HQ x 2: 0.5 M CH3COOH + NaNO3 aq.: 1.0 mL/min: RISB-806 HQColumn Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex OHpak SB-807 HQ, SB-806 HQ: 0.2 M NaCl aq.: 0.5 mL/min: RI MALS (Multi angle light scattering)10710610510410310278Elution volume (mL)Sample : Polyallylamine 0.2 %, 100 µL8.01.0x1081.0x1071.0x1061.0x1054.0Column Eluent Flow rate Detector Column temp. : 40 ˚C1071061051041031021011120.2 M0.1 M0.05 M12.016.0Volume (mL)6.08.0Volume (mL): Shodex OHpak SB-800 HQ series : DMF: 1.0 mL/min: RISB-807 HQ1011Elution volume (mL)0.02 M20.024.0Sample : Polyacrylamide, 100 µLMw: 5,500,00010.012.014.01,500-3,000 cPMn : 201,300Mw : 6,940,500Mw/Mn : 34.47400-800 cPMn : 105,400Mw : 2,126,000Mw/Mn : 20.1850-200 cPMn : 64,600Mw : 402,000Mw/Mn : 6.22From bovine skin Mn : 21,400Mw : 108,100Mw/Mn : 5.05From porcine skinMn : 11,500Mw : 83,700Mw/Mn : 7.28Mn~30,000Mn~50,000Sample : Carboxymethylcellulose 0.1 % each, 50 µL 121015Molecular weight was determined from the calibration curve of pullulan.Sample : 0.1 % each, 100 µLGelatin from bovine skin (Acid treatment, Gel strength : 225 g)Gelatin from porcine skin(Alkali treatment, Gel strength : 90-100 g)1015*Molecular weight was determined from the calibration curve of pullulan.Sample : Cellulose acetate 0.1 % each, 100 µL10Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex OHpak SB-806M HQ x 2: 0.1 M NaCl aq.: 1.0 mL/min: RIColumn Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex OHpak SB-806M HQ x 2: 0.1 M KH2PO4 aq./ 0.1 M Na2HPO4 aq. = 50/50: 1.0 mL/min : RIColumn Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex OHpak SB-806M HQ x 2: 20 mM LiBr in DMF: 1.0 mL/min: RI202530 min202530 min152025minCalibration curves for SB-800 HQ series using pullulan (eluent: H2O)Effects of sodium nitrate in eluent on the analysis of polyallylaminePolyacrylamideCalibration curves for SB-800 HQ series using PEG/PEO (eluent: DMF)CarboxymethylcelluloseCellulose acetateGelatin42
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