4Kll( thgew rauceoM-80nauuPi l)lill0000000000KS-8S0-803KS-802KS145678921132465605125432121354050534 LgandExchange Chromatography CoumnsSaccharides may present their anomers at lower temperatures. By setting the SUGAR series columns at higher temperatures will prevent the anomer separation and this results in providing better chromatograms of each saccharide.GlucoseMannose29 ˚CGlucoseMannose70 ˚CColumn Eluent Flow rate Detector Column temp. : 29 ˚C, 70 ˚C: Shodex SUGAR SC1011: H2O: 0.7 mL/min: RIColumn Eluent Flow rate Detector Column temp. : 80 ˚C: Shodex SUGAR KS-802 x 2: H2O: 1.0 mL/min: RISystem suitabilitysolutionStandard solutionColumn Eluent Flow rate Detector Column temp. : 50 ˚C: Shodex SUGAR SP0810 8C: H2O : 0.7 mL/min : RI (35 ˚C) 1020 min1020 minDp-1Dp-5Dp-1010152. Sorbitol101020 min1020 min1020 min1020 min1020 min1020 minSample : 20 µLHydrolyzed starch 1 %Sample : 5 µL1. Cellobiose 1.0 %1.5 %2. Glucose 0.5 %3. Xylose 0.5 %4. Galactose 0.5 %5. Arabinose 6. Mannose 1.0 %5 20 minSample : 0.1 % each, 20 µL1. Verbascose2. Stachyose3. Raf■nose4. Sucrose5. Pinitol1520min10Sample : 0.5 % each, 10 µL1020 min1020 min5 1015152025Column Eluent Detector Column temp. : 80 ˚C: Shodex SUGAR KS-800 series: H2O: RIColumn Eluent Flow rate Detector Column temp. : 85 ˚C: Shodex SUGAR SP0810: H2O: 0.8 mL/min: RIColumn Eluent Flow rate Detector Column temp. : 80 ˚C: Shodex SUGAR SC1011: H2O: 1.0 mL/min: RI107106105104103102Elution volume (mL)5. chiro-Inositol6. myo-Inositol20minSample : 0.1 % each, 20 µL1. Raf■nose2. Sucrose3. Galactinol4. Galactose5. myo-Inositol30min101112101520min10 minAnalysis of sorbitol according to USP-NF methodSaccharides anomer separationHydrolyzed starchXyloseGalactoseXyloseGalactoseColumn Eluent Flow rate Detector Column temp. : 85 ˚C Column Eluent Flow rate Detector Column temp. : 85 ˚COligosaccharides in soybeanArabinoseArabinose: Shodex SUGAR SP0810: H2O : 0.6 mL/min : RI : Shodex SUGAR KS-802 + KS-801: H2O: 0.6 mL/min: RICalibration curves for KS-800 series using pullulanBiomass sugarsSaccharides related to raffinose biosynthesisPinitol28
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