ili2341120505 1205012345012*34505 ll ll )ilica-basedReversedPhaseChromatography Coumns (ODSCoumns for Sca-basedReversedPhase Chromatography Coumns (ODSCoumns) / SUHPLCSample : 5 µLTryptic digests of Rituximab 0.5 mg/mL10Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex Silica C18M 4E : H2O/CH3CN/CH3OH = 60/10/30: 1.0 mL/min: Fluorescence (Ex. : 365 nm, Em. : 450 nm)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex Silica C18M 4D: (A); H2O/(B); CH3CN Linear gradient; 40 B % to 70 B % (15 min): 1.0 mL/min: UV (280 nm)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex C18U 2D: (A); 0.1 % TFA in H2O/(B); 0.1 % TFA in CH3CN Linear gradient; 10 to 40 B % (0 to 25 min), 40 B % (25 to 30 min), 90 B % (30 to 35 min): 0.2 mL/min: UV (220 nm)Sample : 5 µg/L each, 20 µL1. *Derived A■atoxin G12. *Derived A■atoxin B13. A■atoxin G24. A■atoxin B2*Derivatization with tri■uoroacetic acid101520 minSample : 0.1 mg/mL each, 10 µL1. 6-Gingerol2. 6-Shogaol101515202520 min35min30Column Eluent Flow rate Detector Column temp. : 40 ˚CDeoxynivalenol（100 µg/L）Nivalenol（100 µg/L）Column Eluent Flow rate Detector Column temp. : 40 ˚C734.5 (+)748.5 (+)837.6 (+)749.6 (+)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex Silica C18M 4E : H2O/CH3CN/CH3OH = 90/5/5: 1.0 mL/min: UV (220 nm)Ochratoxin A（1 µg/L）404 > 239 (+)A■atoxin B1（1 µg/L）313 > 241 (+)A■atoxin B2（1 µg/L）315 > 259 (+)A■atoxin G1（1 µg/L）329 > 243 (+)A■atoxin G2（1 µg/L）3-Acetyldeoxynivalenol（100 µg/L）15-Acetyldeoxynivalenol（100 µg/L）331 > 245 (+)397 > 59 (–)355 > 295 (–)371 > 281 (–): Shodex C18U 2B: (A); 10 mM CH3COONH4 aq. /(B); CH3OH Gradient; 0 to 90 B % (0 to 5 min), 90 B % (5 to 7 min), 0 B % (7.01 min), 0 B % (7.01 to 10 min) : 0.4 mL/min: ESI-MS/MS (MRM)ErythromycinClarithromycinRoxithromycin: Shodex C18U 2B: 0.05 % NH3 aq./CH3CN = 40/60 : 0.4 mL/min: ESI-MS (SIM)Sample : 20 µL1. Nivalenol (NIV)2. Deoxynivalenol (DON)*Sample solution of wheat spiked with DON and NIV at 1 g/mL10*: Clarithromycin containing one 13C isotopeAzithromycin15 minSample : 10 µL 6 minSample :10 ng/mL each, 5 µL 6 minGingerol and shogaolTryptic digests of rituximabAflatoxinsLC/MS/MS simultaneous analysis of aflatoxinsLC/MS simultaneous analysis of macrolide antibioticsMycotoxins of trichothecene type25
元のページ ../index.html#27