5min12121205234515061212312053050512345624615312OO050123405l IIliilic InteractionChromatography () Asahpak) Poymer-basedHydrophHLCCoumns (Sample : 40 µg/mL each, 5 µL1. Fructose2. Glucose10Sample : 250 µg/mL each, 20 µL1. α2. γ3. β-Cyclodextrin101. β4. Histidine 6. Nitrate (derived from L-Anserine Nitrate)1015Silica-based amide column(4.6 mm I.D. x 150 mm): Shodex Asahipak NH2P-50 4E Silica based amino column from other manufacturer Silica based amide column from other manufacturer: H2O/CH3CN = 20/80: 1.0 mL/min: Corona charged aerosol Column Eluent Flow rate Detector Column temp. : 30 ˚C (NH2P-50 4E, Silica based amino column) 80 ˚C (Silica based amide column)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex Asahipak NH2P-50 4E: H2O/CH3CN = 40/60: 1.0 mL/min: RIColumn Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex Asahipak NH2P-50 4E: 50 mM NaH2PO4 aq./CH3CN = 40/60: 1.0 mL/min: UV (210 nm)15 min1520min2025minA corona charged aerosol detector measures ef■uents as particles. Thus, the baseline is signi■cantly affected by all components eluted from the column. NH2P series polymer-based amino column eliminates insigni■cant amount of interfering components from the column packing materials. This results in providing stable and low noise baseline.Silica-based amino column(4.6 mm I.D. x 250 mm)NH2P-50 4E（4.6 mm I.D. x 250 mm）Column Eluent Flow rate Detector Column temp. : 30 ˚CEluent Flow rate Detector Column temp. : 40 ˚C: Shodex Asahipak NH2P-50 4E: H2O/CH3CN = 25/75: 1.0 mL/min: UV (210 nm): 40 mM H3PO4 aq./CH3CN = 45/55: 1.0 mL/min: UV (254 nm)Sample : 0.05 % each, 20 µL1. Stevioside2. Rebaudioside A10Sample : 20 µL1. Vitamin B6 50 µg/mL2. Nicotinamide 10 µg/mL3. Vitamin B12 10 µg/mL4. Nicotinic acid 10 µg/mL10 µg/mL5. Folic acid 6. Vitamin C 10 µg/mLColumn Eluent Flow rate Detector Column temp. : 25 ˚C : Shodex Asahipak NH2P-50 4E: H2O/CH3CN = 30/70: 1.0 mL/min: RIColumn Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex Asahipak NH2P-50 4E: H2O/CH3CN = 20/80 : 1.0 mL/min: UV (203 nm)Eluent Flow rate Detector Column temp. : 30 ˚C : 20 mM NaH2PO4 + 30 mM H3PO4 aq. /CH3CN = 20/80: 1.0 mL/min: UV (254 nm)Sample : Fructooligosaccharide syrup 2.5 %, 20 µL1. Fructose2. Glucose3. Sucrose4. 1-Kestose5. Nystose6. 1-Fructofuranosyl-D-nystose10(Sample preparation method)(1) Dissolve 0.1 g of ginseng tea granule in 3 mL of water(2) Add 3 mL of acetonitrile to (1) and mix(3) Filer using a 0.45-µm membrane ■lter and use it as an injection sample.Sample : 10 µL15min10O=CHOCHOCHCHCOHCH2OH152025minStandard solution0.2 mg/mL each 1. Ginsenoside Rg12. Ginsenoside Re3. Ginsenoside Rb1GlucoseGinseng teaSucrose152025minSample : 5 µg/mL each, 10 µL1. Erythorbic acid2. L-Ascorbic acidO=CHOCHOCHCHOCHCH2OH10 minComparison of saccharide analysis using corona charged aerosol detectorCyclodextrinsImidazole dipeptidesSimultaneous analysis of water-soluble vitaminsStevioside and rebaudioside AFructooligosaccharide syrupGinsenosides in ginseng teaAscorbic acid and erythorbic acid23
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