NHNHN0505ONNNO012345678910505l IIlIIilic InteractionChromatography () HLCpak) Poymer-basedHydrophHLCCoumns (HOHOSample : Ribavirin 0.5 ng/mL (in H2O/CH3CN = 1/4), 5 µLNH2HOOHOH10HistamineDp20Enlarged viewOHNH2HOSample : 20 µL0.1 µM each (in H2O)HOOHNHNH2HOHONH2HONH2101520 minSample : 10 µLHydrolyzed dextran 0.5 % (in H2O/CH3CN = 50/50)302530102015202530Sample : 1 µL2.2 mg/mL (in H2O)10 min35min35 minAcarboseMiglitolVogliboseMetformin2 mer (TT)3 mer (TTT)4 mer (GTTT)5 mer (AGTTT)6 mer (AAGTTT)7 mer (GAAGTTT)8 mer (CGAAGTTT)9 mer (GCGAAGTTT)10 mer (AGCGAAGTTT)11 mer (AAGCGAAGTTT)12 mer (TAAGCGAAGTTT)13 mer (TTAAGCGAAGTTT)14 mer (ATTAAGCGAAGTTT)15 mer (GATTAAGCGAAGTTT)16 mer (CGATTAAGCGAAGTTT)17 mer (CCGATTAAGCGAAGTTT)18 mer (ACCGATTAAGCGAAGTTT)19 mer (TACCGATTAAGCGAAGTTT)20 mer (ATACCGATTAAGCGAAGTTT): Shodex HILICpak VN-50 2D: (A); 50 mM HCOONH4 aq./(B); CH3CN Linear gradient; 60 B % (0 to 10 min), 60 B % to 55 B % (10 to 15 min), 60 B % (15 to 20 min): 0.2 mL/min: ESI-MS (SIM)101510Sample : 5 µL5 ng/mL each (in H2O)20 minMonitored ions2 - 4 mer 5 - 11 mer : [M-2H]2-12 - 19 mer : [M-3H]3-: [M-4H]4-20 mer : [M-H]-1520 minLC/MS/MS analysis of monoamine neurotransmittersLC/MS/MS analysis of ribavirinHydrolyzed dextranLC/MS/MS analysis of oral anti-diabetes drugsLC/MS analysis of oligo DNA170 > 107 (+)Noradrenaline184 > 166 (+)Adrenaline154 > 91 (+)DopamineSerotonin177 > 160 (+)112 > 95 (+)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VC-50 2D: (A); 200 mM HCOOH aq./(B); CH3CN Linear gradient (High pressure); 60 B % (0 to 5 min), 60 B % to 10 B % (5 to 6 min), 10 B % (6 to 20 min) : 0.3 mL/min: ESI-MS/MS (MRM)245 > 113 (+)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VC-50 2D: 50 mM HCOOH aq./CH3CN = 10/90: 0.25 mL/min: ESI-MS/MS (MRM)Degree of polymerization (Dp)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VN-50 4D: (A); H2O/(B); CH3CN Linear gradient; 70 B % to 50 B % (0 to 40 min): 1.0 mL/min: Corona charged aerosol646 > 304 (+)208 > 74 (+)268 > 92 (+)130 > 71 (+)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VC-50 2D: (A); 200 mM HCOOH aq./(B); CH3CN Linear gradient (High pressure); 60 B % (0 to 5 min), 60 B % to 20 B % (5 to 6 min), 20 B % (6 to 20 min) : 0.3 mL/min: ESI-MS/MS (MRM)545.1 (–)849.2 (–)1178.2 (–)745.2 (–)901.7 (–)1066.2 (–)1210.7 (–)1375.3 (–)1531.8 (–)1688.0 (–)1226.6 (–)1327.9 (–)1432.3 (–)1542.0 (–)1638.3 (–)1734.7 (–)1839.0 (–)1940.4 (–)1533.3 (–)Column Eluent Flow rate Detector Column temp. : 40 ˚C21
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