NHNHilic Il IIlII1122050531240502468ONN0505nteractionChromatography () HLCpak) Poymer-basedHydrophHLCCoumns (Sample : Aminoglycoside antibiotics 0.1 µg/mL (in H2O), 20 µLSample : 5 µL0.01 µM each (in H2O/CH32. Taurocholic acid10 minSample : 1 ng/mL each, 50 µL101510AMPAEthephonGlyphosateMPPANeomycinAmikacin20 minNH215min: Shodex HILICpak VT-50 2D: 50 mM HCOONH4 aq./CH3CN = 30/70 : 0.2 mL/min: ESI-MS (SIM): Shodex HILICpak VT-50 2D: 25 mM HCOONH4 aq./CH3CN = 80/20: 0.3 mL/min: ESI-MS (SIM)HistamineHistidine: Shodex HILICpak VC-50 2D: 250 mM HCOOH aq./CH3CN = 70/30 : 0.3 mL/min : ESI-MS/MS (MRM) Sample : 100 ng/mL each, 10 µL1. Vitamin B5 (Pantothenic acid) as Calcium pantothenate2. Vitamin C (Ascorbic acid)10Sample : 1 µM each, 5 µL1. Glucose-6-phosphate (G6P)2. Fructose-6-phosphate (F6P)3. Glucose-1-phosphate (G1P)4. Fructose-1-phosphate (F1P)10Sample : 5 ng/mL each (in H2O), 20 µLOH1520 min1214 minNH21015minLC/MS/MS analysis of organic sulfonic acidsLC/MS/MS analysis of glyphosate and glufosinateLC/MS analysis of aminoglycoside antibioticsLC/MS analysis of pantothenic acid and vitamin CLC/MS analysis of phosphorylated saccharidesLC/MS/MS analysis of histamine and histidine20124 > 80 (–)514 > 124 (–)Column Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex HILICpak VT-50 2D: 50 mM HCOONH4 aq./CH3CN = 20/80: 0.3 mL/min: ESI-MS/MS (MRM)110 > 63 (–)(Aminomethylphosphonic acid)180 > 63 (–)Glufosinate143 > 107 (–)168 > 63 (–)151 > 63 (–)(3-Methylphosphinicopropionic acid)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VT-50 2D: 50 mM NH4HCO3 aq./CH3CN = 50/50: 0.3 mL/min: ESI-MS/MS (MRM)97 (–)HSO4-Kanamycin485 (+)Tobramycin468 (+)Gentamicin478 (+)615 (+)586 (+)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VC-50 2D: (A); 1.5 % NH3 aq./(B); CH3CN Linear gradient (High pressure); 30 B % to 10 B % (0 to 5 min), 10 B % (5 to 15 min): 0.3 mL/min: ESI-MS (SIM)220 (+)175 (–)Column Eluent Flow rate Detector Column temp. : 30 ˚C259 (–)Column Eluent Flow rate Detector Column temp. : 60 ˚C156 > 110 (+)Column Eluent Flow rate Detector Column temp. : 40 ˚C112 > 95 (+)
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