( esonnaM fo yrevoceRR1R3R2234230512312NNN3405050505141132405)%0314256l IIlIIilic InteractionChromatography () HLCpak) Poymer-basedHydrophHLCCoumns (R3R2R11234Sample : 5 mg/mL each, 5 µL1. Fructose2. Mannose3. Glucose4. Sucrose101510When an amino column is used for analyzing saccharides, the recovery ratio of reducing saccharides such as mannose, arabinose or xylose is low because the amino group forms Schiff base with reducing saccharides. HILICpak VG-50 is an amino column with improved reducing saccharides' recovery ratios. Improved recovery ratio results in enhancing the sensitivity of the analysis.Silica-based amino column(company B) (4.6 mm I.D. x 150 mm)m/z130 (–)164 (–)148 (–)116 (–)114 (–)203 (–)88 (–)118 (–)Pyruvic acidLactic acidOxalic acidGlyceric acidMalic acidTartaric acidMalonic acidMaleic acid74 (–)104 (–)145 (–)131 (–)154 (–)173 (–)180 (–)146 (–)132 (–)239 (–)meso-ErythritolArabinose + XyloseN-AcetylglucosamineFructose, Mannose, GlucoseGlucosamineSucrose+ LactoseMaltoseGlucuronic acidGalacturonic acid100806040202025 minSample : 5 mg/mL each, 5 µL1. Lactulose2. Epilactose3. Lactose101520 minColumn Eluent Flow rate Detector Column temp. : 50 ˚C: Shodex HILICpak VG-50 4E: H2O/CH3CN/CH3OH = 5/85/10: 0.6 mL/min: RI: Shodex HILICpak VG-50 2D: (A); 0.5 % NH3 aq./(B); CH3CN Linear gradient (High pressure); 80 B % (0 to 2 min), 80 B % to 10 B % (2 to 12 min), 10 B % (12 to 15 min), 80 B % (15 to 20 min) Column Eluent Flow rate Detector Column temp. : 40 ˚C: 0.2 mL/min: ESI-MS (SIM)10Sample : 10 µL, 5 µg/mL each (in H2O/CH3CN = 50/50)No.MelamineAmmelineCyanuric acidAmmelide106. L-Glucose152025minNH2OHOHOHNH2NH2OHOHNH2NH2OHNH21520 minLactose, epilactose, and lactuloseRecovery of reducing sugarSimultaneous analysis of saccharides, organic acids and amino acids with LC/MSRare sugarMelamine and related substancesVG-50 4DSilica-basedamino column(company-A)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VG-50 4D: 30 mM HCOONH4 aq./CH3CN = 35/65 : 0.6 mL/min : Corona charged aerosol m/z87 (–)89 (–)105 (–)VG-50 2D allows simultaneous analysis of saccharides, organic acids and amino acids with LC/MS detection under alkaline conditions. High anionic substances elute under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.133 (–)149 (–)103 (–)115 (–)Citric acid191 (–)121 (–)149 (–)220 (–)179 (–)178 (–)341 (–)Raf■nose503 (–)193 (–)Silica-basedamino column(company-B)15 minSample : 1 µg/mL each (in H2O/CH3CN = 1/4), 5 µL101520 minVG-50 4D（4.6 mm I.D. x 150 mm）Silica-based amino column(company A) (4.6 mm I.D. x 150 mm)Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VG-50 4D Silica based amino columns from other manufacturers: H2O/CH3CN = 20/80: 0.6 mL/min (VG-50 4D) 1.0 mL/min (Silica based amino column): RIColumn Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex HILICpak VG-50 4E : H2O/CH3CN/CH3OH = 5/75/20: 1.0 mL/min: RILeuIlePheMetValProTrpAlaThrGlySerLys + GlnAsnHisArgTyrGluAspCys19
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