Shodex_HPLC_Columns_2023-2024

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1212344356172354680502468051234560501234567821345613245605l l Poymer-basedReversedPhase Chromatography Coumns (RSpak)1520253035Column Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex RSpak DS-413: H2O/CH3CN/THF = 25/45/30: 1.0 mL/min: RIColumn Eluent Flow rate Detector Column temp. : 30 ˚C: Shodex RSpak DM-614: 0.055 M Na2HPO4 + 0.045 M KH2PO4 aq.: 1.0 mL/min: UV (254 nm)Column Eluent Flow rate Detector Column temp. : 40 ˚C : Shodex RSpak NN-814 : 40 mM H3PO4 aq.: 1.0 mL/min : RI Sample : 0.2 % each, 20 µL1. Methyl linoleate2. Methyl palmitate3. Methyl oleate4. Methyl stearate10 minSample : 5 mg/L each (in 2.5 mM H3PO4 aq.), 4 µL1. Vitamin C2. Orotic acid3. Vitamin B64. Vitamin B15. Nicotinamide6. Caffeine10 minSample : 0.1 % each, 20 µL1. Aspartic acid2. Glycine3. Alanine4. Valine5. Methionine6. Isoleucine40455055minColumn Eluent Flow rate Detector Column temp. : 40 ˚C: Shodex RSpak DS-613 : H2O/CH3CN/THF = 30/40/30: 1.0 mL/min: UV (254 nm)Column Eluent Flow rate Detector Column temp. : 30 ˚C83 > 67 (−)62 (−)127 > 111 (−)Column Eluent Flow rate Detector Column temp. : 50 ˚C: Shodex RSpak JJ-50 2D: (A); 200 mM HCOONH4 aq./(B); CH3CN Linear gradient (High pressure); 85 B % (0 to 8 min), 85 B % to 50 B % (8 to 9 min), 50 B % (9 to 14 min), 50 B % to 85 B % (14 to 15 min), 85 B % (15 to 20 min) : 0.3 mL/min: ESI-MS/MS (MRM) for ClO3-, BrO3- ESI-MS (SIM) for NO3-, Br-, Cl-, SO4Sample : 5 µL0.1 %1. m-Cresol 0.1 %2. 2,4-Xylenol 0.5 %3. Benzene 0.5 %4. Toluene 5. Ethylbenzene 0.5 %6. n-Propylbenzene 0.5 % 10 minColumn Eluent 　　　　　　　 Flow rate Detector Column temp. : 40 ˚C: Shodex RSpak DE-413 : 50 mM KH2PO4 + 0.1 % H3PO4 aq./CH3CN: 1.0 mL/min: UV (210 nm): Shodex RSpak DE-213: (A); 0.1 % (v/v) Formic acid aq./(B); CH3CNLinear gradient; 5 B % (0 to 2 min), 5 B % to 15 B % (2 to 2.5 min), 15 B % (2.5 to 10 min): 0.2 mL/min: ESI-MS (SIM)79 (−)35 (−)97 (−)2-Sample : 10 µL1. Saccharin sodium 0.005 %2. p-Hydroxybenzoic acid 0.005 %0.02 %3. Sorbic acid 0.02 %4. Benzoic acid 0.01 %5. Methyl paraben 0.01 %6. Dehydroacetic acid 0.02 %7. Ethyl paraben 8. Propyl paraben 0.02 %Sample : 50 ng/mL each, 10 µLGlyoxylic acidPyruvic acidGlycolic acidLactic acidMaleic acidSuccinic acidFumaric acidAdipic acidSample : 5 µLStandard solution- 0.01 mg/L1. ClO3- 0.1 mg/L2. NO3- 0.01 mg/L3. BrO34. Br- 0.1 mg/L5. Cl- 0.1 mg/L2- 0.1 mg/L6. SO41015101520 min9 min20 min = 65/3573 (–)87 (–)75 (–)89 (–)115 (–)117 (–)115 (–)145 (–)Fatty acid methyl estersAmino acidsVitaminsAlkylbenzenesHigh sensitive analysis of chlorate and bromate by LC/MS/MSFood additives (Preservatives)LC/MS analysis of organic acids17