( endiryp rof rebmun etap laciteroeht evitaeRH2O/CH3CN = 55/45H2O/CH3CN = 55/45H2O/CH3CN = 55/45H2O/CH3CN = 55/45H2O/CH3CN = 55/45H2O/CH3CN = 55/45• Preparative columns [ Preparative columns are made to order. ]H2O/CH3CN = 55/45• Standard columns• Semi-micro columns Poymer-basedReversedPhase Chromatography Coumns (————5555————555—6621512050)%ill00l l )• Provides a large theoretical plate number nearly twice as much as generally available polymer-based reversed phase • Offers enhanced retention of high polar substances compared to ODS columns• Suitable for the analysis of small molecules such as pharmaceuticals in the presence of protein matrix• Ideal for LC/MS analysis of high polar compounds• Fulfills USP-NF L39 requirementsODP2HPODP2 HPChromatograms obtained before and after passing alkaline eluentCorrelation between alkaline eluent passing time and relative theoretical plate numberAnalysis conditionColumn Eluent Flow rate Detector Column temp. : 40 ˚CEluent passing conditions for an alkaline tolerance testColumn Eluent Flow rate Column temp. : 30 ˚CH2O/CH3CN = 55/45H2O/CH3CN = 55/45Features Comparison between ODP2 HP-4D and an ODS column for their alkaline tolerancesODP2 HP-4BODP2 HP-4DODP2 HP-4EODP2 HPG-4AODP2 HP-2BODP2 HP-2DODP2 HPG-2AODP2 HP-10EODP2 HPG-7B(4.6 mm I.D.x150 mm)BeforeAfter 24 hrODS column4.6 x 504.6 x 1504.6 x 2504.6 x 102.0 x 502.0 x 1502.0 x 1010.0 x 250 7.5 x 50: Shodex ODP2 HP-4D ODS column from other manufacturer: 10 mM Sodium phosphate buffer (pH12) /CH3CN = 45/55: 0.6 mL/min12Product CodeProduct NameF7622001F7622002F7622003F6714010Product CodeProduct NameF7622004F7622005F6714011Product CodeProduct NameF6822001F6714015columns doODP2 HP-4D(4.6 mm I.D.x150 mm)BeforeAfter 500 hr: Shodex ODP2 HP-4D ODS column from other manufacturer: H2O/CH3OH = 70/30: 1.0 mL/min: UV (254 nm)Plate Number(TP/column) ≥ 3,500≥ 10,000≥ 17,000(guard column)Plate Number(TP/column)≥ 3,000≥ 7,000(guard column)Plate Number(TP/column)≥ 9,500(guard column)Sample : 5 L1. Pyridine 200 g/mL2. Phenol 430 g/mL10minParticle SizeFunctional Group(µm)Functional GroupParticle Size(µm)Particle Size(µm)10minPore SizeColumn Size (mm)(Å)I.D. x Length404040Base Material: PolyhydroxymethacrylatePore SizeColumn Size (mm)(Å)I.D. x Length4040Base Material: PolyhydroxymethacrylateColumn Size (mm)I.D. x LengthBase Material: Polyhydroxymethacrylate12010080604020200Alkaline eluent passing time (hr)Shipping SolventShipping SolventShipping SolventODP2 HP-4DODS column400600Polymer-based Reversed Phase Chromatography Columns (ODP2 HP)
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