尿中のアスコルビン酸
GS-320 7EはGS-320 HQの旧タイプカラムです。
Sample : Urine
1. Glucose
2. Diketoglonic acid
3. Diketogluconic acid
4. Dehydroisoascorbic acid
5. Dehydroascorbic acid
6. Ascorbic acid
7. Isoascorbic acid
Column : Shodex Asahipak GS-320 7E (7.5mmID*250mm)
Eluent : The eluent was prepared by dissolving 4.5g of tartaric acid,
1.5g of EDTA-2Na and 1g of beta-thiodiglycol in 2L of ultrapure water and
adjusting the pH to 3.0 with 4M sodium hydroxide solution.
Flow rate : 1.0mL/min
Detector : Fluorescence (Ex. 325nm, Em. 400nm) (Using post-column reaction)
Column temp. : 30℃
T.saki et al., J.Chromatogr., 385 (1987) 287.