| Substance | Elution Volume (mL) | ||
|---|---|---|---|
|
DE-613
|
KC-LG
+ KC811 x 2 |
KC-LG
+ DE-613 + KC-811 x 2 |
|
| Oxalic acid |
3.0
|
10.5
|
13.5
|
| Maleic acid |
4.2
|
11.4
|
15.6
|
| Phosphoric acid |
3.0
|
11.4
|
14.4
|
| α-Ketoglutaric acid |
3.2
|
11.5
|
14.7
|
| Pyruvic acid |
4.0
|
12.3
|
16.3
|
| Citric acid |
5.0
|
12.3
|
17.3
|
| D-Glucuronic acid |
3.3
|
12.3
|
15.6
|
| Isocitric acid |
5.0
|
12.6
|
17.6
|
| Tartaric acid |
3.8
|
13.0
|
16.8
|
| Gluconic acid |
3.4
|
13.5
|
16.9
|
| Malonic acid |
5.3
|
13.6
|
18.9
|
| trans-Aconitic acid |
15.7
|
13.7
|
29.4
|
| Glyoxylic acid |
3.7
|
14.0
|
17.7
|
| Malic acid |
5.1
|
14.1
|
19.2
|
| Succinic acid |
8.6
|
16.8
|
25.2
|
| Glycolic acid |
4.3
|
17.0
|
21.3
|
| Lactic acid |
5.5
|
17.5
|
23.0
|
| Itaconic acid |
15.7
|
17.6
|
33.3
|
| Fumaric acid |
16.9
|
17.8
|
34.7
|
| β-Hydroxypropionic acid |
5.0
|
18.3
|
23.3
|
| Formic acid |
4.7
|
18.7
|
23.4
|
| Uric acid |
6.9
|
19.4
|
26.3
|
| Acetic acid |
6.3
|
20.4
|
26.7
|
| Mesaconic acid |
43.4
|
20.7
|
64.1
|
| Adipic acid |
30.2
|
21.1
|
51.3
|
| Levulinic acid |
11.5
|
21.2
|
32.7
|
| L-Pyroglutamic acid |
5.5
|
21.4
|
26.9
|
| Propionic acid |
12.8
|
23.9
|
36.7
|
| CO32-,HCO3- |
7.8
|
25.7
|
33.5
|
| Isobutyric acid |
32.3
|
26.5
|
58.8
|
| n-Butyric acid |
32.6
|
28.5
|
61.1
|
| Isovaleric acid |
Not eluted
|
32.9
|
Not eluted
|
| n-Valeric acid |
Not eluted
|
39.7
|
Not eluted
|
Column : Shodex RSpak DE-613 (6.0mmID*150mm)
Shodex RSpak KC-LG (8.0mmID*50mm) + KC-811 (8.0mmID*300mm) x 2
Shodex RSpak KC-LG (8.0mmID*50mm) + DE-613 (6.0mmID*150mm) + KC-811 (8.0mmID*300mm) x 2
Eluent : 2mM HClO4 aq.
Column temp. : 50℃