| Substance | Elution Volume (mL) | |||||
|---|---|---|---|---|---|---|
|
30℃
|
40℃
|
50℃
|
60℃
|
70℃
|
80℃
|
|
| Oxalic acid |
9.66
|
9.63
|
9.62
|
9.56
|
9.51
|
9.47
|
| Maleic acid |
10.74
|
10.65
|
10.51
|
10.53
|
10.47
|
10.41
|
| Phosphoric acid |
11.01
|
11.14
|
11.27
|
11.41
|
11.53
|
11.70
|
| α-Ketoglutaric acid |
11.08
|
10.95
|
10.87
|
10.77
|
10.70
|
10.64
|
| 2-Ketogluconic acid |
11.56
|
11.52
|
11.52
|
11.47
|
11.44
|
11.42
|
| Glucuronic acid |
11.67
|
11.69
|
11.71
|
11.69
|
11.68
|
*
|
| Pyruvic acid |
11.91
|
11.82
|
11.76
|
11.72
|
11.70
|
11.71
|
| Citric acid |
11.93
|
11.81
|
11.71
|
11.57
|
11.46
|
11.36
|
| Oxalacetic acid |
12.00
|
11.86
|
11.77
|
11.76
|
11.73
|
11.70
|
| Isocitric acid |
12.14
|
11.99
|
11.88
|
11.72
|
11.60
|
11.48
|
| Tartaric acid |
12.36
|
12.23
|
12.14
|
11.99
|
11.90
|
11.81
|
| Galacturonic
acid |
12.48
|
12.44
|
12.41
|
12.31
|
12.26
|
12.20
|
| Gluconic acid |
12.79
|
12.78
|
12.78
|
12.76
|
12.90
|
12.91
|
| Malonic acid |
13.26
|
13.09
|
12.96
|
12.81
|
12.71
|
12.61
|
| trans-Aconitic
acid |
13.99
|
13.46
|
13.11
|
12.74
|
12.47
|
12.24
|
| Glyoxylic acid |
13.30
|
13.27
|
13.26
|
13.19
|
13.14
|
13.09
|
| Malic acid |
13.69
|
13.51
|
13.37
|
13.19
|
13.04
|
12.91
|
| Ascorbic acid |
14.13
|
13.99
|
13.88
|
13.72
|
13.59
|
*
|
| α-Hydroxygltaric acid |
14.58
|
14.25
|
14.05
|
13.83
|
13.98
|
13.91
|
| Succinic acid |
16.53
|
16.11
|
15.79
|
15.40
|
15.10
|
14.82
|
| Glycolic acid |
16.55
|
16.39
|
16.26
|
16.06
|
15.89
|
15.74
|
| Lactic acid |
16.83
|
16.75
|
16.67
|
16.51
|
16.37
|
16.21
|
| 2-Hydroxyisobutyric
acid |
17.02
|
17.06
|
17.05
|
16.94
|
16.82
|
16.66
|
| β-Hydroxypropionic acid |
17.53
|
17.34
|
17.18
|
16.96
|
16.76
|
16.57
|
| Itaconic acid |
17.83
|
17.26
|
16.80
|
16.29
|
15.87
|
15.48
|
| Formic acid |
18.23
|
18.03
|
17.86
|
17.64
|
17.46
|
17.28
|
| Fumaric acid |
19.23
|
18.02
|
17.16
|
16.39
|
15.79
|
15.30
|
| Acetic acid |
19.92
|
19.66
|
19.41
|
19.11
|
18.82
|
18.55
|
| Uric acid |
20.71
|
19.28
|
18.31
|
17.40
|
16.75
|
16.22
|
| Levulinic acid |
22.06
|
21.07
|
20.27
|
19.53
|
18.87
|
18.27
|
| Pyroglutamic acid |
22.91
|
21.80
|
20.99
|
20.33
|
19.77
|
19.29
|
| Adipic acid |
23.10
|
21.41
|
20.17
|
19.03
|
18.11
|
17.34
|
| Propionic acid |
23.53
|
23.02
|
22.54
|
22.01
|
21.49
|
20.69
|
| Mesaconic
acid |
24.02
|
21.93
|
20.47
|
19.15
|
18.14
|
17.31
|
| CO32-,HCO3- |
24.29
|
24.48
|
24.50
|
24.51
|
24.35
|
24.15
|
| Isobutyric acid |
27.10
|
26.35
|
25.59
|
24.77
|
23.95
|
23.14
|
| n-Butyric acid |
29.76
|
28.66
|
27.61
|
26.53
|
25.49
|
24.48
|
| Isovaleric acid |
35.65
|
33.92
|
32.28
|
30.60
|
29.00
|
27.46
|
| n-Valeric acid |
45.05
|
41.77
|
38.89
|
36.12
|
33.60
|
31.27
|
Columns : Shodex RSpak KC-811 (8.0mmID*300mm) x 2 Eluent : 3mM HClO4 aq. Flow rate : 1.0mL/min