糖の保持容量

7種類の糖分析用カラムSUGAR SP0810、SC1011、KS-801、SZ5532、SC1211、Asahipak NH2P-50 4E、GS-220 HQを用いて代表的な糖の溶出容量を示しました。　 この表では、ピークトップの溶出容量しか分かりませんので、実際のピークの重なり具合を見たい場合には、糖分析用カラムのシミュレーションをご覧ください。

Substances
 Elution Volume (mL)
SUGAR Asahipak
SP0810 SC1011 KS-801 SZ5532 SC1211 NH2P-50 4E GS-220 HQ x 2
Pb2+ Ca2+ Na+ Zn2+ Ca2+
N-Acetyl-α
-D-glucosamine		 8.86 7.75 6.68 - 4.10 6.66
D(+)-Arabinose 10.42 8.91 8.21 5.11 5.56 6.18
D-Arabitol 15.86 11.33 7.63 7.27 8.16 6.29
Aspartame - - - - 34.02 -
2-Deoxy-D-glucose 8.83 7.58 7.15 4.34 4.02 6.02
Difructose anhydride III 7.07 6.30 5.81 4.30 * 7.77
Dulcitol 20.18 12.76 7.40 9.46 11.28 7.45
meso-Erythritol 12.70 10.09 7.86 5.73 6.27 5.43 17.38
Ethanol 11.13 11.33 10.09 - 4.27 -
1-Fructofuranosyl
-D-nystose		 6.05 5.27 4.76 31.43 * - 14.24
D(-)-Fructose 11.05 8.85 7.71 5.37 5.90 6.75 17.05
D(+)-Fucose 10.48 8.84 8.09 4.50 4.96 5.43
D(+)-Galactose 9.74 7.98 7.58 6.46 4.98 8.10 16.60
4'-Galactosyllactose 7.42 6.02 5.40 19.02 * 21.66
α-D-Galacturonic acid - 6.28 4.36 - 5.63 -
Gentiobiose 7.22 6.08 5.75 10.50 * 16.36
Glucose 8.63 7.30 7.17 5.87 4.76 8.61 16.75
Glycerol





17.83
Glycyrrhizin - - - - 2.71 -
myo-Inositol 12.77 8.86 7.99 12.63 7.87 9.96
Isomaltose 7.68 6.26 5.95 10.57 * 15.18 15.57
Isomaltotriose 7.09 5.75 5.34 21.17 * 27.55 14.72
1-Kestose 6.79 5.75 5.26 13.09 * 20.11 15.31
Kojibiose 7.56 6.21 5.88 9.65 * 14.82
Lactitol 13.27 8.09 6.13 16.35 6.67 11.82 15.50
Lactose 8.05 6.51 5.99 10.12 4.07 13.27 15.71
Lactosylfructoside 7.12 5.88 5.29 14.69 * 18.98
Lactulose 9.13 6.99 6.19 9.16 4.65 10.72
Maltitol 12.23 8.26 6.03 13.04 6.77 11.82 15.82
Maltoheptaose





14.82
Maltohexaose





14.56
Maltopentaose





14.31
Maltose 7.85 6.34 5.94 8.67 * 14.24 16.11
Maltotriose 7.48 5.89 5.38 13.79 * 24.96 15.56
Mannitol 15.80 11.10 7.23 8.75 9.03 7.39
D-Mannose 10.72 8.17 7.64 5.83 5.01 7.84
D(+)-Melezitose 6.94 5.79 5.24 13.60 * 19.27
Melibiose 8.16 6.45 5.98 11.69 4.23 14.70
Methyl-α
-D-mannopyranoside		 11.13 8.87 7.78 3.99 4.39 4.71
Nystose 6.38 5.45 4.93 20.05 * 31.90 14.72
Palatinit 2peaks 2peaks 5.90 2peaks 2peaks 12.73
Palatinose 7.84 6.45 5.89 8.08 3.99mL 12.12
Panose 7.14 5.78 5.32 16.87 * 25.60
L-Phenylalanine - - - - 31.58 -
D(+)Raffinose 7.14 5.78 5.29 16.36 * 20.25 15.08
D(+)-Rhamnose 9.77 8.23 7.37 3.93 4.43 5.52
D(-)-Ribose 19.35 13.66 9.04 4.82 8.64 5.45
Rutinose 7.81 6.49 5.80 6.65 * 10.87
Saccharin sodium 6.96 5.44 4.33 6.77 * 5.68
D(-)-Sorbitol 21.61 13.31 7.42 9.79 11.88 7.09 16.60
D(+)-Sorbose 9.67 8.03 7.38 5.12 4.92 7.35
Stachyose 6.82 5.57 4.97 - * 36.22 14.33
Stevioside - - - 4.14 * 6.07
Sucrose 7.54 6.29 5.87 7.91 * 11.87 16.26
α-D-Talose 21.33 12.59 8.76 5.69 8.51 6.47
Theanderose 2peaks 2peaks 2peaks 2peaks * 2peaks
Trehalose 7.62 6.27 5.78 10.85 * 13.25
Trehalulose 8.92 6.95 6.10 9.54 4.78 11.68
Xylitol 19.87 13.14 7.94 7.77 10.16 6.10 16.88
Xylobiose 8.16 6.68 6.40 5.65 * 9.05
D(+)-Xylose 9.21 7.09 7.71 4.55 4.48 6.58 17.46
D-Xylulose 10.64 9.02 8.04 4.06 5.07 5.41

(-)→検出せず (*)→溶媒ピークと重なる
Columns      : Shodex SUGAR SP0810, SC1011, KS-801 (8.0mmID*300mm each)
Eluent       : H2O
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 80℃

Column       : Shodex SUGAR SZ5532 (6.0mmID*150mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 60℃

Column       : Shodex SUGAR SC1211 (6.0mmID*250mm)
Eluent       : H2O/CH3CN=65/35
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 70℃

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30℃

Column       : Shodex Asahipak GS-220 HQ (7.5mmID*300mm) x 2
Eluent       : H2O
Flow rate    : 0.5mL/min
Detector     : Shodex RI
Column temp. : 60℃