| Pittcon 2008 |
Liquid Chromatography: Pharmaceutical Applications
3/6 Thursday Morning
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| (2730-16P) Novel High Performance SEC Column for Water Soluble Polymer |
Ryuji Takahashi, Masatoshi Murakami (Shodex, Showa Denko KK)
Ritsuko Ohno (Shodex Service Lab)
Ken Tsengo (Shodex, Showa Denko America Inc.) |
Shodex has developed a new OHpak SB400 series HPLC column for GFC analysis. It is a further improvement over
the conventional SB-800 series. The packing material is polyhydroxymethacrylate gel and it has controlled surface
hydrophilicity and pore distribution. The columns can be used with alkaline solution up to pH10, and it can be used
with water soluble organic solvents like methanol, acetonitrile and DMF.
We have developed packing material with smaller particle size and downsized columns. As the result, the new column can handle
small amount of sample while obtaining higher performance than conventional SB-800 series. SB400 series have four kinds of
molecular weight ranges. These exclusion limits range from 1,000, to 1,000,000. Particularly, the lowest molecular range (1,000)
is not included in the conventional type of SB-800 series. This brand new column can be applied to analyze water soluble substances
with MW less than 1,000. We will introduce basic features of SB400 series and their applications.
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Liquid Chromatography / Mass Spectrometry: Pharmaceutical Applications
3/4 Tuesday Morning |
| (1240-1P) LCMS analysis of pharmaceutical molecules and their metabolites in blood plasma by novel polymer-based column for reversed phase chromatography |
Junji Sasuga (Analysis and Physical properties center, Showa Denko K.K.)
Kei Oide (Shodex Service Lab)
Eiji Kagawa, Hideyuki Kondo (Shodex, Showa Denko KK)
Ken Tsengo (Shodex, Showa Denko America Inc.) |
We have developed a novel polymer-based column for reversed phase chromatography. The new column has better retention of
hydrophilic substances and it is not susceptible to protein adsorption that causes ion suppression. We applied the column in
the LCMS analysis of pharmaceutical molecules in biological fluid.
With the new column, we obtained accurate results with
the analysis of Barbital (5ng) in blood after SPE pretreatment. With the general ODS columns, an underestimation (70%, 3.5ng)
occurred. The new column generates good data with low salt concentration and without the use of ion pair reagents.
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Liquid Chromatography: Environment
3/6 Thursday Morning
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| (2720-6P) Development of solid phase extraction method with use of activated carbon fiber |
Akiko Sinoda, Yuko Iio, Etsuo Fujimoto (Shodex, Showa Denko KK)
Ken Tsengo (Shodex, Showa Denko America Inc.) |
Solid phase extraction using chemically synthesized activated carbon is widely used for environmental and biological
analysis. Especially, activated carbon is used to capture high polar chemicals which can not be captured by C18 and polystyrene
beads.
The synthesized activated carbon columns are ideal for the large-scale removal of saccharides in bio-ethanol production
process. The conventional activated carbon is not suitable for this application because they are packed with particle-type
activated carbon. The particles clog up the column causing low flow rate. Furthermore, some particle-type activated carbon
can contaminate the final product, requiring an extra step of filtering process to remove them.
We have developed a new solid phase extraction method with a new phenol-type activated carbon fiber cartridge.
This method has the advantages of a rapid flow speed without clogging and good recoveries of high polar chemicals.
In our poster, we demonstrate the basic performance and advantages, application data such as saccharide concentration.
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