Amides (3) (DE-413)

In order to separate hydrophilic compounds, water or 100% buffer solution is used as the eluent, however, such eluent usually shorten the column life of ODS columns. Since the packing material of RSpak DE-413 is chemically stable and has long column life. The stable analysis results can be obtained using DE-413 even when 100% aqueous solution is used as the eluent.


Sample : Amides
1. 2micro-L/mL Formamide
2. 2.40mg/mL Acetamide
3. 2.16mg/mL Propionamide

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : 10mM H3PO4 aq.
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 50deg-C