Brij® 56 (Polyoxyethylene(10) Cetyl Ether)
Polyoxyethylene(10) cetyl ether (Brij
® 56) which is a nonionic surfactant was analyzed using Asahipak
GF-310 HQ (an Aqueous/Organic (multi-solvent use) SEC column).
The component of which polyethylene chain is longer elutes faster.

Sample :
Brij® 56, Polyoxyethylene(10) cetyl ether 1%, 20μL
Column : Shodex Asahipak GF-310 HQ (7.5mmID*300mm)
Eluent : CH3CN/H2O=60/40
Flow rate : 0.6mL/min
Detector : Shodex RI
Column temp. : 40°C