Simultaneous Analysis of Anionic and Non-ionic Surfactants

OHpak SB-802.5 HQ, an aqueous solvent SEC (GFC) column, was used to analyze an anionic surfactant (sodium dodecylbenzenesulfonate) and non-ionic surfactant (triton X-100) simultaneously. Owing to the ion exclusion mode, anionic surfactant elutes at first and it is separated from non-ionic surfactant.


Sample : 100μL
(A) Sodium dodecylbenzenesulfonate(Soft type)
50mg/L
(B) Triton X-100 (Octylphenol ethoxylate) 1000mg/L

Column       : Shodex OHpak SB-802.5 HQ (8.0mmID*300mm)
Eluent       : H2O/CH3CN=70/30
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 60°C