Albumin Analysis (Comparison of Multimode Column with GFC Column)
Asahipak GS-520 7G
, a multimode column, separates proteins
by GFC mode. However, serum albumins such as bovine and human albumins are eluted
slowly because the albumins have affinity with the molecular structure of poly(vinyl
alcohol) gel which is packed in GS-520 7G. Therefore, albumins can separate with
components of similar molecular weight. Here, albumin is separated with tranferrin,
IgG, IgM and IgA. With PROTEIN KW-800
and transferrin cannot be separated because their molecular weights are too close
to be separated by GFC mode.
GS-520 7G is an old type of GS-520 HQ.
Columns : Shodex Asahipak GS-520 7G (7.5mmID*500mm) x 4
Eluent : 0.1M Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate : 1.0mL/min
Detector : UV(250nm)
Column temp. : 30°C