Carbonate and Other Anions (2) (NI-424)

IC NI-424 has high theoretical plate number (more than 5,000). Carbonate and other anions can be separated well with NI-424.


Sample : 50micro-L
1. HCO₃^- 40.0mg/L
2. H₂PO₄^- 4.0mg/L
3. F^- 0.3mg/L
4. Cl^- 0.5mg/L
5. NO₂^-1.2mg/L
6. Br^- 2.0mg/L
7. NO₃^- 2.0mg/L
8. SO₄^2- 2.0mg/L

Column       : Shodex IC NI-424 (4.6mmID*100mm)
Eluent       : *18mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris + 2mM Phenylboronic acid + 0.005mM *2CyDTA(pH4.2)
Flow rate    : 1.0mL/min
Detector     : Shodex CD
Column temp. : 40deg-C

^*1: The prescribed pH can be obtained by mixing the reagents in the composition described here.
^*2: CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid