Influence of Temperature on Retention Time (SI-52 4E)

Please use this column with suppressor type ion chromatography system.

In analysis using IC SI-52 4E, the retention times of some anions are very sensitive to the column temperature. An example is showed in the figure below. Bromide (Br^-) and chlorate (ClO₃^-) are well separated at 45 deg-C or over, while the separation of chlorite (ClO₂^-) and bromate (BrO₃^-) are improved with lowering temperature.


Sample : 100micro-L
1. F^- (2mg/L)
2. ClO₂^- (1mg/L)
3. BrO₃^- (2mg/L)
4. Cl^- (10mg/L)
5. NO₂^- (5mg/L)
6. Br^- (10mg/L)
7. ClO₃^- (1mg/L)
8. NO₃^- (30mg/L)
9. PO₄^3- (15mg/L)
10. SO₄^2- (40mg/L)

Column       : Shodex IC SI-52 4E (4.0mmID*250mm)
Eluent       : 3.6mM Na2CO3 aq.
Flow rate    : 0.8mL/min
Detector     : Suppressed conductivity