Anions in milk were analyzed using IC I-524A
. Milk contains
a considerable amount of proteins and fats, and therefore should be pretreated.
100-fold dilution with pure water was made and then treated by an solid phase
extraction cartridge for reversed phase chromatography.
Sample : 100-fold diluted Milk
Sample : Anion Standards, 50μL
Column : Shodex IC I-524A (4.6mmID*100mm)
Eluent : 1.5mM p-Hydoxybenzoic acid + 1.7mM N,N-Diethylethanolamine + 10% CH3OH
Flow rate : 1.5mL/min
Detector : Non-suppressed coductivity
Column temp. : 40°C