River Water (1) (I-524A)

Anions in river water were analyzed using IC I-524A. In river water, small volume of H₂PO₄^-, F^- and Br^- as well as Cl^-, NO₃^- and SO₄^2- are present. This eluent can be used to separate H₂PO₄^- and F^-.

(Preparation of eluent)
1) Put 0.332g of reagent grade phthalic acid, 0.223g of reagent grade Tris(hydroxymethyl)aminomethane and 18.5g of boric acid into a 1L of messflask.
2) Dilute with purified water to make up a 1 liter solution.
3) Dissolve by stirring and warming.
4) Pass through a 0.2micron membrane filter before use.

This method is patented in Japan by Shimadzu Corporation.


Sample : River water(Water)
1. H₂PO₄^-
2. F^-
3. Cl^-
4. Br^-
5. NO₃^-
6. SO₄^2-

Column       : Shodex IC I-524A
Eluent       : 2.0mM Phthalic acid + 1.84mM Tris(hydroxymethyl)aminomethane + 300mM Boric acid(pH4.0)
Flow rate    : 1.2mL/min
Detector     : Shodex CD
Column temp. : 40deg-C