Anions in Beer

The eluent used here is suitable for the simultaneous analysis of inorganic anions and dibasic and tribasic organic acids using IC I-524A. Organic acids are contained in beer and they interfere with the quantification of anions. Cl^-, NO₃^- and SO₄^2- can be separated without such interference of organic acids. Organic acids, oxalic acid and citric acid also can be analyzed without interference.

(Preparation of eluent)
1) Put 0.249g of reagent grade phthalic acid, 0.171g of reagent grade tris(hydroxymethyl)aminomethane and 18.5g of boric acid into a 1 liter messflask.
2) Dilute with purified water to make up a 1 liter solution.
3) Dissolve by stirring and warming.
4) Pass through a 0.2micron membrane filter before use.

This method is patented in Japan by Shimadzu Corporation.


Sample : Beer
1. Acetic acid
2. H₂PO₄^-, Succinic acid, Pyroglutamic acid
3. Lactic acid, Pyruvic acid
4. Cl^-
5. Malic acid, Br^-
6. NO₃^-
7. Oxalic acid
8. Citric acid
9. SO₄^2-
10. Tartaric acid

Column       : Shodex IC I-524A (4.6mmID*100mm)
Eluent       : 1.5mM Phthalic acid + 1.38mM Tris(hydroxymethyl)aminomethane + 300mM Boric acid(pH4.0)
Flow rate    : 1.2mL/min
Detector     : Shodex CD
Column temp. : 40deg-C