HEETs Produced by Polymorphonuclear Leukocyte of Rat

HEETs produced by polymorphonuclear leukocyte of rat were analyzed using Asahipak ODP-50 6D ( a column for reversed phase chromatography ).


Sample : 5-HETE

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : H2O/CH3COOH/CH3CN/CH3OH=50/0.1/50/10
Flow rate    : 1.0mL/min
Detector     : UV(235nm)
Column temp. : 30deg-C

Courtesy of Dr. Oishi and Dr. Yamaki, Kitazato University