is an improved version of OHpak M-414, which was recommended by a Japanese standard, "Methods of Analysis in Health Science" (2005). The total quantity of vitamin B1
using RSpak NN-614 with a phosphate buffer eluent. The vitamin B1
separated by the column is converted into thiochrome using a postcolumn
reagent and detected by a fluorescence detector.
Sample : Thiamine hydrochloride
Column : Shodex RSpak NN-614 (6.0mmID*150mm)
Eluent : 45mM KH2PO4 + 55mM Na2HPO4 aq.
Reagent : 0.01% K3Fe(CN)6 + 15% NaOH
Flow rate : (Eluent); 0.7mL/min, (Reagent); 0.7mL/min
Detector : Fluorescence detector(Ex.: 375nm, Em.: 450nm)
Column temp. : 25°C