Procainamide in Serum
This shows one advantage of using an alkaline eluent for the analysis of procainamide
in serum such as procainamide. Procainamide cannot be separated from other serum
components at pH 3 or 7. However, since procainamide, which is one of a tertiary
amine, dissociates a little at pH11, in consequence, it is strongly retained by
the packing material of Asahipak ODP-50 4D
( a column for
reversed phase chromatography ) and can be separated from other serum components.
As explained above, for the analysis of basic drugs which have tertiary
amino groups, an alkaline eluent at pH11 is effective.
1) Dissolve procainamide in CH3
2) Put 10 μL of the above in the centrifuge tube and blow dry it.
3) Add 1,000 μL of the serum and stir.
4) Add 3,000 μL of CH3
CN and stir.
5) Centrifuge (3,500rpm , 20min) skim and filtrate by using a 0.45μm filter
to make a sample.
6) Inject 20 μL of the sample.
Sample : Procainamide in serum
Column : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
Eluent : 25mM Phosphate buffer/CH3CN=75/25
Flow rate : 0.6mL/min
Detector : UV(254nm)
Column temp. : 30°C