Basic Drugs : Under Neutral Condition
Elution patterns of four kinds of basic drugs which have tertiary amine are shown.
Under the eluent conditions, large peak tailing and delay of elution are observed
for ODS-A and ODS-B except scopolamine. To the contrary, the elution patterns
of ODS-C (polymer-coated) and ODP (polymer-based) for procainamide and atropine
are far better than those of ODS-A and ODS-B though peak tailing is observed for
diphenhydramine. These chromatograms show non-specific adsorption which is usually observed for
columns and its solution using polymer-based or polymer-coated columns.
Columns : Shodex Asahipak ODP-50 4D (4.6mmID*150mm), ODS-A, ODS-B, ODS-C (4.6mmID*150mm each)
Eluent : 25mM Phosphate buffer(pH7)/CH3CN=60/40
Flow rate : 0.6mL/min
Detector : UV(254nm)
Column temp. : 30°C