Methylxantines (1)
Methylxantines were analyzed using RSpak
DS-613 (a column for reversed phase chromatography).

Sample :
1.
Xanthine
2.
Theobromine
3.
Theophylline
4.
Caffeine
Column : Shodex RSpak DS-613 (6.0mmID*150mm)
Eluent : H2O/CH3CN=88/12
Flow rate : 1.0mL/min
Detector : UV(254nm)
Column temp. : 40deg-C