Guidelines for Shodex Column Selection : Drugs, Metabolites and Chiral Compounds
For the analysis of drugs and metabolites, reversed phase mode, ion exchange mode and multimode can be used. For such analysis, polymer-based columns such as the Asahipak ODP, RSpak DE and DS series are well- suited because they can be used even with a strong alkaline eluent. For the analysis of basic drugs such as small amines, the above polymer- based columns are especially suited because these columns can be used with an eluent of pH10-11. At such conditions, they has almost the same result as analysis with an ion pair reagent yet avoids the problems caused by the use of the latter.
(Chiral Compounds)
Shodex columns are available in two modes for chiral separation. ORpak CDBS-453 column is packed with silica-based gel bonded with cyclodextrin derivatives as ligands. The separation mode is based on the difference in the degree of inclusion of the samples into the cyclic structure of the ligand. The ORpak CRX-894 column is packed with a polymer-based gel to which L-amino acid derivative is bonded.
