Phosphorylated Saccharides (2)
Glucose can be anearobically decomposed into lactic acid and ethanol.This decomposing process is called as glucolytic pathway.
In this pathway, some products such as glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate, etc.
are produced as intermediary products. Here, fructose-6-phosphate and fructose-1,6-bisphosphate are analyzed using
Asahipak NH2P-50 4E
by ion-exchange mode.
Sample : 5 mg/mL each, 20μL
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : 300mM Sodium phosphate buffer(pH4.4)
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 40°C