Yogurt: Sugar added (NH2P-50)
When analyzing saccharides in food, it is necessary to remove the substances besides
saccharides before analysis. This procedure is called as sample pre-treatment. Here, the analysis result of saccharides in food using Asahipak NH2P-50 4E
is shown together with the sample pre-treatment procedure.
1) Take 5g of sugar added yogurt in a beaker, add 30mL of pure water and neutralize
with 10%(w/v) NaOH.
2) Apply ultrasonic vibration for 30 minutes and make 50 mL solution.
3) Filtrate with No.5B paper filter and then with 0.45μm membrane
The comparison of NH2P-50 column and silica-based amino column for the analysis of yogurt(sugar added) is as follows:
1)The peaks of fructose and galactose cannot be detected using silica-based amino column.
2)In case of NH2P-50 column, the peak of lactose is lager compared with the case
of silica-based amino column. This result shows the fact that most of lactose
is adsorbed by silica-based amino column. Since the adsorption of lactose by NH2P-50
column is not so much, the detection limit of NH2P-50 column is lower than that
of silica-based amino column.
Sample : Yogurt
Columns : Shodex Asahipak NH2P-50 4E, Amino column from other manufacturer (4.6mmID*250mm each)
Eluent : CH3CN/H2O=75/25
Flow rate : 0.6mL/min (NH2P-50), 1.0mL/min (Amino column from other manufacturer)
Detector : Shodex RI
Column temp. : 25°C