Purine Bases and Pyrimidine Bases (3)
Six components were completely separated using RSpak
DE-613 (a column for reversed phase chromatography). Analysis time can be shortened by adding acetonitrile in the eluent.

Sample :
1.
Cytosine
2.
Uracil
3.
Hypoxanthine
4.
Guanine
5.
Thymine
6.
Adenine
Column : Shodex RSpak DE-613 (6.0mmID*150mm)
Eluent : 0.05M KH2PO4 aq.
Flow rate : 1.0mL/min
Detector : UV(254nm)
Column temp. : 25deg-C