Adenine Nucleotides in Red Blood Cell

With Asahipak GS-320 HQ (a multimode column), proteins and nucleic acid components of high molecular weights are separated by the GFC mode, they elute all together at the Vo position. One of the advantages of GS-320 HQ is, for samples consisting of low molecular weight compounds coexisting with those of high molecular weight, that the low molecular weight compounds can be analyzed directly without removing high molecular weight compounds such as proteins.


Sample :
1. Hemoglobin
2. ATP
3. ADP
4. AMP

Column       : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent       : 100mM Sodium phosphate buffer(pH7.0) + 300mM NaCl
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 30deg-C