Bases, Nucleosides and Nucleotides

Asahipak GS-320 HQ (a multimode column) is suitable not only for the separation of purine bases, pyrimidine bases, nucleosides and nucleotides but also for the separation of samples of their mixtures. GS-320 HQ permits one-time separation by isocratic elution without using gradient elution.


Sample :
1. ATP
2. ADP
3. AMP
4. IMP
5. GMP
6. Adenine
7. Adenosine
8. Inosine
9. Guanine
10. Hypoxanthine

Column       : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent       : 200mM Sodium phosphate buffer(pH3.0)
Flow rate    : 0.6mL/min
Detector     : UV(260nm)
Column temp. : 30deg-C