Calibration Curve for GS-520 (Proteins)
A calibration curve for Asahipak GS-520 7G
(a multimode column) is shown. When an eluent with a relatively high salt concentration is used for Asahipak GS series, substances are eluted in decreasing order of
molecular weight as it is the GFC mode which function mainly. GS-520, which has a large pore size, can analyze proteins with
molecular weight up to 1,000,000. Samples such as human and bovine serum albumins which exhibit strong affinity with
the molecular structure of the packing material are eluted belatedly.
Making use of this characteristics, GS-520 is used exclusively for the analysis of serum albumin.
GS-520 7G is an old type of GS-520 HQ.
7. Chymotrypsinogen A
10. Cytochrome c
Column : Shodex Asahipak GS-520 7G (7.5mmID*500mm)
Eluent : 0.1M Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate : 1.0mL/min
Detector : UV(210nm)
Column temp. : Room temp.