Proteins in Human Serum (1) (QA-825)
Proteins in Human serum were analyzed using IEC QA-825
( a column for strong anion exchange chromatography ).
In this analysis, the sample was separated into four peaks including the transferrin
peak; the largest one is serum albumin.
Sample : Human serum
4. Serum albumin
Column : Shodex IEC QA-825 (8.0mmID*75mm)
Eluent : (A); 20mM Tris-HCl buffer(pH8.6)
(B); (A) + 0.5M NaCl
Linear gradient: 0min to 60min, 100% (A) to 50% (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.