Guidelines for Shodex Column Selection : Proteins, Peptides and Amino Acids
For the analysis of proteins, peptides and amino acids, a variety of Shodex columns
are available using different separation modes such as SEC, reversed phase (RP), ion
exchange (IEC), hydrophobic interaction chromatography (HIC) and multimode.
Aqueous SEC (GFC) columns are suitable for the first screening of unknown samples, whereby substances
elute in decreasing order of molecular weight. Reversed phase columns which are
most widely used separate by substances by the difference of hydrophobicity. For
the selection of the most effective column, it is also important to set up the
right conditions for the eluent according to the sample charasteristics.