Calibration Standards for SEC

When size exclusion chromatography (GPC or GFC) is performed, it is necessary to make a calibration curve.
It is recommended that Shodex STANDARD be used to obtain the calibration curve. The molecular weight range of Shodex STANDARD are very narrow and molecular weights are measured accurately. Five types of standards are available.

[Polyctyrene(PS)]
SL-105
SM-105
SH-75		 · For organic SEC(GPC)
· High linearity polystyrene with anionic polymerization
· Soluble in tetrahydrofuran(THF), chloroform, toluene, and o-dichlorobenzene

Product name Contents MW range
STANDARD SL-105
0.5g x 10 kinds
500 to 22,000
STANDARD SM-105
0.5g x 10 kinds
1,200 to 3,800,000
STANDARD SH-75
0.5g x 7 kinds
590,000 to 7,500,000
SL-105 SM-105 SH-75
Std.No. Mp Mw/Mn Std.No. Mp Mw/Mn Std.No. Mp Mw/Mn
S-20
S-11
S-6.9
S-5.0
S-4.4
S-2.9
S-1.9
S-1.2
S-1.0
S-0.5
 19,800
10,700
6,930
5,030
4,430
2,900
1,930
1,200
1,050
580
 1.02
1.02
1.03
1.03
1.03
1.03
1.05
1.07
1.07
1.12		 S-3730
S-2480
S-1230
S-579
S-197
S-55.1
S-31.4
S-12.8
S-3.95
S-1.20
 3,730,000
2,480,000
1,230,000
579,000
197,000
55,100
31,400
12,800
3,950
1,200
 1.04
1.05
1.05
1.02
1.02
1.03
1.02
1.02
1.03
1.07		 S-7450
S-3790
S-3250
S-2220
S-1820
S-1060
S-591



 7,450,000
3,790,000
3,250,000
2,220,000
1,820,000
1,060,000
591,000



 1.07
1.05
1.04
1.03
1.04
1.03
1.03

[Structure formula of S series]

Note: Molecular weights (Mw/Mn and Mp) may vary depending on production lots even in the same type of Shodex STANDARD.

[Polymethylmethacrylate(PMMA)]
M-75 · For organic SEC(GPC)
· Narrow molecular weight range
· Soluble in hexafluoroisopropanol(HFIP) and dimethylformamide(DMF)

Product name Contents MW range
STANDARD M-75
0.5g x 7 kinds
1,800 to 950,000
M-75
Std.No. Mp Mw/Mn
M-949
M-451
M-139
M-53
M-21
M-7.1
M-1.9
 949,000
451,000
139,000
52,600
20,800
7,100
1,890
 1.05
1.02
1.05
1.02
1.03
1.08
1.10
Note: Molecular weights (Mw/Mn and Mp) may vary depending on production lots even in the same type of Shodex STANDARD.

[Pullulan]
P-82 · For aqueous SEC(GPC)
· Has no side chains
· High solubility in water eliminates the possibility of recrystallization

Product name Contents MW range
STANDARD P-82
0.2g x 8 kinds
5,000 to 800,000
P-82
Std.No. Mw Mw/Mn Mp
P-800
P-400
P-200
P-100
P-50
P-20
P-10
P-5		 788,000
404,000
212,000
112,000
47,300
22,800
11,800
5,900
1.23
1.13
1.13
1.12
1.06
1.07
1.10
1.09
708,000
375,000
200,000
107,000
47,000
21,100
11,100
5,900

[Structure formula of P series]

Note: Molecular weights (Mw, Mw/Mn and Mp) may vary depending on production lots even in the same type of Shodex STANDARD.