Eluent Conditions for Asahipak GF, GS HQ
As the eluent for Asahipak GS-HQ
series and GF-HQ
series, either organic solvent
or aqueous solution
can be used.
Please refer to Multimode Columns
and Aqueous/Organic SEC Columns
Please refer to the followings:
1. Strong electrolytic salts
Total salts concentration should be 0.5 M or less. Aqueous solutions of NaCl,
and other such salts may be used as eluents. Phosphoric acid, acetic acid, citric
acid, boric acid, and tris-HCI buffer solutions may also be used, either independently
or in combination with these salts.
2. Aqueous uric acid and guanidine hydrochloride
These solutions are widely used as protein
denaturalization agents. As their concentration
is generally high, it is recommended that
they be used in columns set aside exclusively
for this purpose, as their frequent interchange
with eluents of lower concentration would
tend to shorten the column service life.
3. Use of surfactants
Aqueous solutions containing SDS, Brij-35 or other surfactants can also be used
as eluents. The eluent interchange time following the use of such an eluent will
generally be longer than for ordinary eluents. Approx. 50% methanol may be used
to shorten the replacement time.
4. Solutions with high salt content
After passage of a high-concentration salt solution, its removal from the flow
lines by flushing with water is sometimes necessary to prevent salt precipitation
at the pump and the injector. In such cases, column life is best preserved by
first removing the column, before flushing the LC system flow lines.