Features of Multimode Columns (Nucleic Acid Analysis)
Asahipak GS-320 HQ
is a column which operates basically
in SEC mode. However, depending on the eluent conditions, it allows separation
by multiple modes combining reversed phase and ion exchange modes. The column
is particularly effective in analyzing samples such as proteins and nucleic acid
components, which are made up of both acidic and basic components. In this separation
mode, samples are eluted in the order of acidic, neutral and basic. Separation
can also be adjusted by changing the eluent components.
1) When a neutral/acidic eluent is used.
|| As it is the ion exclusion mode which is in operation, substances
are eluted relatively quickly.
|| As substances are separated by reversed phase mode, they
are eluted moderately.
|| As it is the ion exchange mode which is in operation, substances
are eluted relatively slow.
2) When a basic eluent is used.
|| Basically, substances are excluded together at the Vo position
but, with acidic samples of large molecular weights, there are cases in
which substances are retained in the column.
|| Substances are separated by reversed phase mode.
|| If the eluent has a higher pH than the pKa of the sample,
substances are separated by reversed phase mode as the dissociation of basic
samples is suppressed.