Eluent Conditions for KW400-4F and PROTEIN KW-800
Eluent conditions for KW400-4F series and KW-800 series columns
1) Typical eluent
The following solutions are generally used
as the eluent.
Phosphate buffer solution
Tris hydrochloric solution
Acetate buffer solution
2) Salt to be added
Salts such as Na2
are usually added to the above mentioned buffer solutions. The suitable salt concentration
is 0.1 to 0.3M.
(1) The pH range of the eluent should be 3.0 to 7.5 range.
(2) Use of salts containing chlorine ions should preferably be avoided, as chlorine
ions are corrosive to the column or piping of the instrument.
3) Use of urea or guanidlne hydrochloride
Urea or 6M guanidine hydrochloride solutions,
which are often used as modifier of proteins,
can be used as the eluent.
Since the replacement of the eluent takes
a fairly long time, it is recommended to
use one column specifically for such an eluent.
4) Use of surfactant
Aqueous solutions of surfactant such as SDS
and Brij can also be used.
5) Use of polar organic solvent
Polar organic solvents, such as acetonitrile,
methanol and ethanol
can also be used as the eluent.